Sonja STURM

Dr. rer. nat. Mag. pharm.

Assistenzprofessorin

 

Institute of Pharmacy, Pharmacognosy

address: Josef-Moeller-Haus, Innrain 52, A-6020 Innsbruck
e-mail: sonja.sturm@uibk.ac.at
phone: +43-512-507-5305
fax: +43-512-507-2939

Research interests

Development and validation of chromatographic and capillaryelectrophoretic techniques for the determination of pharmacologically relevant natural products in phytopharmaceuticals and medicinal plants

Coupling of high performance analytical methods (capillaryelectrophoresis, high performance liquid chromatography) with mass-spectrometry

Isolation and characterization of pharmacologically active natural compounds

Papers

PhD thesis abstract

 

ZUR CHEMIE, ANALYTIK UND PHARMAKOLOGIE VON OXINDOL-ALKALOIDEN AUS UNCARIA TOMENTOSA UND ZUR KAPILLAR-ELEKTROPHORETISCHEN ANALYSE DER HAUPT-ALKALOIDE AUS ESCHSCHOLTZIA CALIFORNICA

 

Phytochemical analysis of the rootbark of Uncaria tomentosa (Willd.) DC. (Rubiaceae) resulted in the isolation of ten oxindole alkaloids which were identified as Isopteropodine, Pteropodine, Isomitraphylline, Uncarine F, Mitraphylline, Speciophylline, Pteropodinic acid, Isopteropodine-N-oxide, Uncarin F-N-oxide and Formosanine-N-oxide. The structure elucidation was performed by UV-, IR-, NMR-spectroscopy and Mass-spectrometry.

A HPLC- and a Capillary electrophoresis (CE) -method were developed for the qualitative and quantitative analysis of the main alkaloids in crude U. tomentosa extrakts and phytopharmaceuticals. By HPLC the best seperation was achieved with an acetonitrile/methanol and phosphate buffer (pH 6.6, 60-30 % in 30 min) solvent gradient and a column temperature of 15 °C. By Capillary electrophoresis the best separation was achieved using a 20 mM phosphate running buffer (pH 5.6) at a constant voltage of 10 kV. Furthermore a capillary electrophoretic method for the analysis of the main alkaloids of Eschscholtzia californica Cham. (Papaveraceae) was developed. The best separation was achieved using a citric acid/phosphat running buffer (ionic strength 45.2 mmol/l, pH 2.8) and a constant voltage of 20 kV and a temperature of 10 °C.

The alkaloids from Uncaria tomentosa were tested for their antileucemic effects on the cell lines HL 60 and U-937. The measurment of the antiproliferative effect on those cell lines was performed with a colorimetric (MTT) and a clonogenic agar assay. The most pronounced effect was found for Uncarine F.

 

Supervisor: Professor Dr. Hermann Stuppner

C. Hödl, K. Raunegger, R. Strommer, G.F. Ecker, O. Kunert, S. Sturm, C. Seger, E. Haslinger, R. Steiner, W.S. Strauss, H.W. Schramm

Syntheses and Antigestagenic Activity of Mifepristone Derivatives.

J. Med. Chem. 52, 1268–1274 (2009) DOI link

A.N. Assimopoulou , S. Sturm, H. Stuppner, V.P. Papageorgiou

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high-speed counter-current chromatography.

Biomed. Chromatogr. 23, 182-198 (2009). DOI link

Z Li, S. Sturm, B. Svejda, H. Höger, E. Schraml, E. Inglic, V. Siegl, H. Stuppner, R. Pfragner

Anticancer activity of novel extracts from Cautleya gracilis (Smith) Dandy: apoptosis in human medullary thyroid carcinoma cells.

Anticancer Res. 28, 2705-2713 (2008). 

S. Sturm, J. Schinnerl, H. Greger, H. Stuppner

NACE-ESI-IT-MS analysis of pyrrolo- and pyrido[1,2-a]azepine alkaloids in Stemona

Electrophoresis, 29, 2079-2087 (2008)  DOI link

Z Li, S. Sturm, H. Stuppner, E. Schraml, V Aguiriano-Moser, V Siegl, R. Pfragner

The dichloromethane fraction of Stemona tuberosa Lour inhibits tumor cell growth and induces apoptosis of human medullary thyroid carcinoma cells.

Biologics: targets & therapy, 1, 455-463 (2007)  Link

C. Seger, S. Sturm

HPLC-SPE-NMR - a novel hyphenation technique. (Review)

LC-GC Europe, 20, 587-597 (2007) Link

S. Sturm, C. Seger, M. Godejohann, M. Spraul, H. Stuppner

Conventional sample enrichment strategies combined with high-performance liquid chromatography–solid phase extraction–nuclear magnetic resonance analysis allows analyte identification from a single minuscule Corydalis solida plant tuber.

J. Chromatogr. A, 1163,138-144 (2007) DOI link

S. Sturm, C. Seger, H. Stuppner

Analysis of Central European Corydalis species by nonaqueous capillary electrophoresis-electrospray ion trap mass  spectrometry.

J. Chromatogr. A, 1159, 42-50 (2007) DOI link

C. Seger, S. Sturm

Analytical aspects of plant metabolite profiling platforms - current standings and future aims. (Review)

J. Proteome Res. 6, 480-497 (2007) DOI link

C. Seger, K. Eberhart, S. Sturm, H. Strasser, H. Stuppner

Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography, High yield strategy for structurally closely related analytes - destruxin derivatives from Metarhizium anisopliae as a case study

J. Chromatogr. A, 1117, 67-73 (2006) DOI link

S. Schwaiger, C. Seger, B. Wiesbauer, P. Schneider, E. Ellmerer, S. Sturm, H. Stuppner

Development of a HPLC-DAD/MS assay for the Identification and Quantification of major phenolic Edelweiss (Leontopodium alpium Cass.) constituents.

Phytochem Anal., (2006) 17, 291-298 (2006) DOI link

S. Sturm, E.M. Strasser, H. Stuppner

Quantification of Fumaria officinalis isoquinoline alkaloids by nonaqueous capillary electrophoresis–electrospray ion trap mass spectrometry

J. Chromatogr. A 1112, 331-338 (2006) DOI link

N. Bacher, M. Tiefenthaler, S. Sturm, H. Stuppner, M.J. Ausserlechner, R. Kofler, G. Kowalinka

Oxindole alkaloids from Uncaria tomentosa induce apoptosis in proliferating, G0/G1-arrested and bcl-2-expressing acute lymphoblastic leukaemia cells.

Brit. J. Haematol.,132, 615-622 (2006) DOI link

J. Rollinger, J. Ewelt, C. Seger, S. Sturm, E. Ellmerer, H. Stuppner

New insights into the acetylcholinesterase inhibitory activity of Lycopodium clavatum L..

Planta Med., 71, 1040-1043 (2005) DOI link

C. Seger, S. Sturm, E. Haslinger, H. Stuppner

NMR assignment of 22-deoxocucurbitacin D and cucurbitacin D from Ecballium elaterium L. (Cucurbitaceae).

Monatsh. Chem.,136, 1645-1649 (2005) DOI link

 M. Ganzera, D. Piereder, S. Sturm, C. Erdelmeier, H. Stuppner

Urtica dioica Agglutinin - Separation, identification and quantitation of individual isolectins by capillary electrophoresis and capillary electrophoresis - mass spectrometry.

Electrophoresis, 26, 1724-1731 (2005) DOI link

C. Seger, S. Sturm, M.E. Mair, E. Ellmerer, H. Stuppner

1H and 13C NMR signal assignment of cucurbitacine derivatives from Citrullus colocynthis (L.) Schrader and Ecballium elaterium L. (Cucurbitaceae)

Magn. Reson. Chem., 43, 489-491 (2005) DOI link

C. Seger, S. Sturm, T. Längle, W. Wimmer, H. Stuppner, H. Strasser

Development of a sensitive HPLC-DAD assay for the detection and quantification of the fungal metabolite oosporein from fungal culture broth and biological pest control formulations.

J. Agri. Food Chem., 53, 1364 - 1369 (2005) DOI link

C. Seger, M. Godejohann, L.H. Tseng, M. Spraul, A. Girtler, S. Sturm, H. Stuppner

The LC-DAD-MS/SPE-NMR hyphenation: A tool for the analysis of pharmaceutically used plant extracts: Identification of isobaric iridoid glycoside regioisomers from Harpagophytum procumbens.

Anal. Chem., 77, 878-885 (2005) DOI link

C. Seger, S. Sturm, H. Stuppner, T.M. Butt, H. Strasser

Combination of a new sample preparation strategy and an accelerated high-performance liquid chromatography assay with photodiode array and mass spectrometric detection for the determination of destruxins from Metarhizium anisopliae culture broth.

J. Chromatogr. A 1061, 35-43 (2004) DOI link

C. Seger, S. Sturm, E.M. Strasser, E. Ellmerer, H. Stuppner

1H and 13C NMR signal assignment of benzyl-isoquinoline alkaloids from Fumaria officinalis L. (Papaveraceae)

Magn. Reson. Chem. 42, 882-886 (2004) DOI link

C. Seger, H. Römpp, S. Sturm,  E. Haslinger, P.C. Schmidt, F. Hadacek

Characterization of supercritical fluid extracts of St. John's Wort (Hypericum perforatum L.) by HPLC-MS and GC-MS.

Eur. J. Pharm. Sci., 21, 453-463 (2004) DOI link

C. Seger, S. Sturm, E. Haslinger, H. Stuppner

A new cucurbitacin D related 16,23-epoxy derivative and its isomerization products.

Org. Lett., 6, 633-636 (2004) DOI link

S. Sturm, V. Taglioli, A.R. Bilia, F. Vinceri, H. Stuppner

Analysis of alkaloids in Ipecacuanhae radix and preparations by capillary zone electrophoresis.

J. Sep. Sci., 26, 1175-1179 (2003) DOI link

C. Zidorn, E.P. Ellmerer, S. Sturm, H. Stuppner

Tyrolobibenzyls E and F from Scorzonera humilis and distribution of caffeic acid derivatives, lignans and tyrolobibenzyls in European taxa of the subtribe Scorzonerinae (Lactucaceae, Asteraceae).

Phytochemistry, 63, 61-67 (2003) DOI link

C. Zidorn, S. Sturm, J.W. Dawson, J.W. vanKlink, H. Stuppner, N.B. Perry

Chemosystematic investigations of irregular diterpenes in Anisotome and related New Zealand Apiaceae.

Phytochemistry, 59, 293-304 (2002) DOI link

S. Sturm, H. Stuppner

Analysis of iridoid glycosides from Picrorhiza kurroa  by capillary electrophoresis and high performance liquid chromatography - mass Spectrometry.

Chromatographia, 53, 612-618 (2001)

Abstract: A micellar electrokinetic chromatog. (MEKC) method was applied to the anal. of iridoid glycosides of Picrorhiza kurroa Royle Ex Benth. Baseline sepn. was achieved within 16 min using a fused silica capillary and a borate buffer soln. (100 mM, pH 8.60) contg. 30 mM SDS and 1% acetonitrile. The applied voltage was 25 kV, the thermostating temp. was kept const. at 30°C. Injection was performed in the pressure mode for 2 s, the detection wavelength was 205 nm. For optimization of the CE method an iridoid-contg. fraction of a methanolic ext. of P. kurroa was injected. The impact of the electrolyte compn., the pH value, the ionic strength, the concn. of SDS, the influence of org. additives, the voltage and the temp. on resoln. of adjacent peaks was studied. The optimized method was used for quant. detn. of the main iridoids in crude methanolic exts. of P.kurroa. Anal. was also performed by HPLC-MS using an electrospray ionization (ESI) interface. Conditions were optimized both for efficient HPLC and for the MS system. Mass spectra of all HPLC peaks showed one dominant signal corresponding to [M+Na]+. The good agreement of quant. CE results with those obtained by LC clearly demonstrated the applicability of the presented methods

C. Zidorn, E.P. Ellmerer, K.H. Ongania, S. Sturm, H. Stuppner

New taxonomically significant sesquiterpenoids from Leontodon autumnalis.

J. Nat. Prod., 63, 812-816 (2000) DOI link

S. Sturm, H. Stuppner

Analysis of cucurbitacins in medicinal plants by liquid chromatography mass spectrometry.

Phytochem. Anal., 11, 121-127 (2000) DOI link

S. Sturm, H. Stuppner

Analysis of flavonols of Sedum telephium L. leaves by capillary electrophoresis and HPLC-mass spectrometry.

Chromatographia, 50, 433-438 (1999)

Abstract: Flavonol glycosides of Sedum telephium L. were analyzed by MEKC. Baseline sepn. was achieved within 14 min using a fused silica capillary and a borate/phosphate buffer soln. (35 mM, pH 5.8) contg. 35 mM SDS and 4% MeOH. The applied voltage was 25 kV and the thermostating temp. was kept const. at 40°. Injection was performed via the pressure mode for 3 s, the detection wavelength was 205 nm. The optimized MEKC method was used for the quant. detn. of flavonol glycosides in exts. of S. telephium leaves. Anal. was also performed by HPLC-MS using an electrospray ionization (ESI) interface. The good agreement between the quant. CE results and those obtained by LC clearly demonstrated the applicability of the methods presented.

S. Sturm, H. Stuppner

Analysis of isoquinoline alkaloids in medicinal plants by capillary electrophoresis – mass spectrometry.

Electrophoresis, 19, 3026-3032 (1998)

Abstract: The technique of capillary electrophoresis - mass spectrometry (CE-MS) was applied for determination of isoquinoline alkaloids in crude methanolic extracts of medicinal plants. For the CE separations ammonium formate buffer solutions (70 or 100 mM, pH 3.0 or 4.0) containing 10% methanol or 20-60% acetonitrile as additives were used. The applied voltage was 25 kV, the thermostating temperature was kept constant at 25 degrees C. Coupling with the mass spectrometer was performed via an atmospherical pressure ionization (API) interface and the electrospray ionization technique (ESI). As sheath liquid 5 mM formic acid in acetonitrile at a flow rate of 3 microL/min was used. The spray voltage was 4.5 kV and the temperature of the heated capillary was chosen to be 200 degrees C. Detection in the positive ionization mode resulted in mass spectra showing either the molecular ions [M]+ or the protonated molecular ions [M+H]+. The presented method allows detection and identification of isoquinoline alkaloids in crude methanolic extracts of medicinal plants as Eschscholzia californica CHAM. (Papaveraceae), Hydrastis canadensis L. (Ranunculaceae), Berberis vulgaris L. (Berberidaceae), Jateorhiza palmata (LAM.) MIERS (Menispermaceae) and Chelidonium majus L. (Papaveraceae).

M. Ganzera, S. Sturm, H. Stuppner

HPLC/MS and MECC Analysis of Coumarins.

Chromatographia, 46, 197-203 (1997)

T. Hung, S. Sturm, H. Stuppner

TLC and HPLC/MS analysis of the gum resins of Ailanthus grandis and related species.

Sci. Pharm., 64, 471-478 (1996)

H. Stuppner, S. Sturm

HPLC/MS and CZE of dianthrones from Cassia angustifolia and acutifolia.

Chromatographia, 42, 697-704 (1996)

S. Sturm, R.R. Gil, H.B. Chai, O.D. Ngassapa, T. Santisuk, V. Reutrakul, A. Howe, M. Moss, J.M. Besterman, S.L. Yang, J.E. Farthing, R. Murraytait, J.A. Lewis, M.J. O’Neill, N.R. Farnsworth, G.A. Cordell, J.M. Pezzuto, A.D. Kinghorn

Lupane derivatives from Lophopetalum walichii with farnesyl protein transferase inhibitory activity.

J. Nat. Prod., 59, 658-663 (1996) DOI link

H. Stuppner, S. Sturm, N. Mulinacci, F. Vinceri

Capillary zone electrophoretic analysis of the main alkaloids from Eschscholtzia californica.

Chromatographia, 37, 579-584 (1993)

S. Sturm, H. Stuppner, G. Kowalinka

HPLC analysis of  the main oxindole alkaloids from Uncaria tomentosa.

Chromatographia, 34, 597-600 (1992)

S. Sturm, H. Stuppner, G. Kowalinka

Capillary electrophoretic analysis of oxindole alkaloids from Uncaria tomentosa.

J. Chromatogr. A., 609, 375-380 (1992)

Abstract: The main oxindole alkaloids from the root bark of Uncaria tomentosa were separated by capillary electrophoresis. The electrophoretic parameters for the separation of the six alkaloids were optimized by studying the impact of the buffer composition, the ionic strength and the pH and the applied electric field on the separation efficiency of the analytical system. The best separation was achieved with a fused-silica capillary tube and 20 mM phosphate running buffer (pH 5.6) at a constant voltage of 10 kV. The determination of oxindole alkaloids in a crude methanolic extract of Uncaria tomentosa demonstrates the applicability of this method.

Selected Posters

K. Eberhart, C. Seger, S. Sturm, H. Strasser, H. Stuppner

53rd GA Congress 21 - 25 August 2005 Florence, Italy

High yield isolation of destruxins A, B, D, E, and E-diol, the major depsipeptide derivatives of Metarhizium anisopliae by combining lipophilic Sephadex gel chromatography and HSCCC

M. Godejohann, C. Seger, L.H. Tseng, M. Spraul, A. Girtler, S. Sturm, H. Stuppner

  Joint Meeting DPhG, ÖPhG and CPS, 6-9 October 2004, Regensburg, Germany

Identification of iridoid glycoside regioisomers from Harpagophytum procumbens using a LC-DAD-MS/SPE-NMR hyphenation as dereplication tool

C. Seger, W. Wimmer, S. Sturm, H. Stuppner, H. Strasser

  15th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2004) 2-6 May 2004, Florence, Italy

Method development for the trace analysis of the highly polar fungal hydroxybenzoquinone derivative oosporein.

C. Seger, S. Sturm, H. Stupner, H. Strasser

  15th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2004) 2-6 May 2004, Florence, Italy

A rapid method for the isolation and analysis of destruxins from Metarhizium anisopliae culture broth.

C. Seger, S. Sturm, H. Römpp, P.C. Schmidt, E. Haslinger, F. Hadacek

   7th Symposium on Instrumental Analysis, 21 September - 24 September 2003, Pecs, Hungary

Characterization of lipophilic plant constituents with LC-MS and GC-MS - Hypericum perforatum L. supercritical fluid extracts as a case study.

S. Sturm, C. Seger, M. Lanzinger, G. Abel, M. Popp, H. Stuppner

   51st GA Congress 31 August - 4 September 2003 Kiel,Germany

A new cucurbitacin D related 16,23-epoxy derivative from Ecballium elaterium L. fruit juice and it’s isomerization products.

C. Seger, D. Erlebach, U. Griesser, W. Wimmer, S. Sturm, H. Stuppner, H. Strasser

    1st FEMS Congress 29 June - 2 July 2003 Lubljana, Slovenia

Risk assessment of fungal biological control agents: Beauveria brongniartii and its metabolite oosporein - needs and deeds.

 
 

Sonja Sturm, 2009-03-28

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