PD Dr. Christoph SEGER

 

e-mail: christoph.seger@uki.at
phone: +43-512-504-81155

 

 

 

 

LECTURE 2010

          Link to documents

 

 

Overview research interests

 

Application of LC-MS/MS in clinical routine and research

             Immunosuppressant TDM

             Assay development

Application of hyphenated NMR methods in phytoanalysis.

             online LC-SPE-NMR

          Metabolic profiling

 

 

Publications since 2005

 

Reviews

S. Sturm, C. Seger

Liquid chromatography-nuclear magnetic resonance coupling as alternative to liquid chromatography-mass spectrometry hyphenations: Curious option or powerful and complementary routine tool?

   J Chromatogr A 2012, in press

M. Vogeser, C. Seger

Pitfalls associated with the use of liquid chromatography-tandem mass spectrometry in the clinical laboratory.

   Clin. Chem., 56, 1234-1244 (2010)

C. Seger, M. Vogeser

Immunosuppressant drug monitoring – a routine undertaking?

   J. Lab. Med.., 34, 117-128 (2010)

M. Vogeser, C. Seger

A decade of HPLC-MS/MS in the routine clinical laboratory - goals for further developments.

   Clin. Biochem. 41, 649-662 (2008)

C. Seger, S. Sturm

HPLC-SPE-NMR - a novel hyphenation technique.

   LC-GC Europe20, 587-597 (2007)

C. Seger, A. Griesmacher

Some important aspects of implementing tandem mass spectrometry in a routine clinical laboratory environment.

   Biochem. Med. 17, 29-51 (2007)

C. Seger, S. Sturm

Analytical aspects of plant metabolite profiling platforms - current standings and future aims.

   J. Proteome Res. 6, 480-497 (2007)

E. Holmes, H. Tang, Y. Wang, C. Seger

The assessment of plant metabolite profiles by NMR based methodologies.

   Planta Med., 72, 771-785 (2006)

 

Clinical Chemistry & Medicinal Chemistry

B.E. Seeber, S.C. Ziehr, A. Gschließer, C. Moser, V. Mattle, C. Seger, A. Griesmacher, N. Concin, H. Concin, L. Wildt

Quantitative levonorgestrel plasma level measurements in patients with regular and prolonged use of the levonorgestrel-releasing intrauterine system.

  Contraception., 2012, in press

M. Vogeser, C. Seger

LC-MS/MS in clinical chemistry (Editorial).

   J Chromatogr B 883-884, 1-2 (2012)

C. Moser, D. Zoderer, G. Luef, M. Rauchenzauner, L. Wildt, A. Griesmacher, C. Seger

Simultaneous online SPE-LC-MS/MS quantification of six widely used synthetic progestins in human plasma.

  Anal. Bioanal. Chem. 403, 961-972 (2011)

S. Weiler, G. Falkensammer, C. Seger, M. Joannidis, R. Bellmann

Teicoplanin pharmacokinetics during albumin dialysis.

  Artif. Organs 35, 969-971 (2011)

C. Moser, D. A. Gschliesser, V. Mattle, L. Wildt, A. Griesmacher, C. Seger

An ultra-sensitive online SPE-LC-MS/MS method for the quantification of levonorgestrel released from intrauterine devices.

  Anal. Bioanal. Chem. 400, 2655-2662 (2011)

B.E. Seeber, T. Czech, H. Buchner, K.T. Barnhart, C. Seger, G. Daxenbichler, L. Wildt, H. Dieplinger

The vitamin E–binding protein afamin is altered significantly in the peritoneal fluid of women with endometriosis.

  Fertil. Steril., 94, 2923-2926 (2010)

R. Bellmann, G. Falkensammer, C. Seger, S. Weiler, J. Kountchev, M. Joannidis

Teicoplanin pharmacokinetics in critically ill patients on continuous veno-venous hemofiltration.

  Int. J. Clin. Pharmacol. Ther, 48, 243-249 (2010)

C. Seger, K. Tentschert, W. Stöggl, A. Griesmacher, S. Ramsay

A rapid HPLC-MS/MS method for the simultaneous quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human blood samples.

   Nat. Protoc., 4, 526-534 (2009)

U. Reisinger, S. Schwaiger, I. Zeller, B. Messner, R. Stigler, D. Wiedemann, T. Mayr, C. Seger, T. Schachner, V.M. Dirsch, A.M. Vollmar, J.O. Bonatti, H. Stuppner, G. Laufer, D. Bernhard

Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts.

   Cardiovasc. Res., 82, 542-549 (2009)

C. Hödl, K. Raunegger, R. Strommer, G.F. Ecker, O. Kunert, S. Sturm, C. Seger, E. Haslinger, R. Steiner, W.S. Strauss, H.W. Schramm

Syntheses and Antigestagenic Activity of Mifepristone Derivatives.

   J. Med. Chem. 52, 1268–1274 (2009)

K. Hochegger, G.L. Jansky, A. Soleiman, A.M. Wolf, A. Tagwerker, C. Seger, A. Griesmacher, G. Mayer, A.R. Rosenkranz

The mTOR-inhibitor rapamycin in experimental anti-GBM glomerulonephritis – friend or foe?

   J. Am. Soc. Nephrol. 19, 1520-1529 (2008)

C. Buchta, C. Bittner, H. Heinzl, P. Höcker, M. Macher, M. Mayrhofer, R. Schmid, C. Seger, M. Dettke

The plasticizer di(2-ethylhexyl)phthalate (DEHP) in stored plateletpheresis concentrates: Exposure of patients transfused and reducing measures.

   Transfusion, 45, 798-802 (2005)

 

Phytoanalysis and Phytochemistry

V. Pieri, S. Sturm, C. Seger, C. Franz, H. Stuppner

1H NMR-based metabolic profiling and target analysis: a combined approach for the quality control of Thymus vulgaris.

   Metabolomics, 8, 335-346 (2012)

B. Lukaseder, S. Vajrodaya, T. Hehenberger, C. Seger, M. Nagl, G. Lutz-Kutschera, W. Robien, H. Greger, O. Hofer.

Prenylated flavanones and flavanonols as chemical markers in Glycosmis species (Rutaceae).

   Phytochemistry. 70, 1030–1037 (2009)

C. Seger, S. Pointinger, H. Greger, O. Hofer

Isoeichlerianic acid from Aglaia silvestris and revision of the stereochemistry of foveolin B.

   Tetrahedron Lett, 49, 4313-4315 (2008)

S. Sturm, C. Seger,  M. Godejohann, M. Spraul, H. Stuppner

Conventional sample enrichment strategies combined with high-performance liquid chromatography–solid phase extraction–nuclear magnetic resonance analysis allows analyte identification from a single minuscule Corydalis solida plant tuber.

   J. Chromatogr. A, 1163,138-144 (2007)

S. Sturm, C. Seger,  H. Stuppner

Analysis of Central European Corydalis species by nonaqueous capillary electrophoresis-electrospray ion trap mass spectrometry.

   J. Chromatogr. A, 1159, 42-50 (2007)

C. Seger, M. Godejohann, M. Spraul, H. Stuppner, F. Hadacek

Reaction product analysis by high-performance liquid chromatography–solid-phase extraction–nuclear magnetic resonance: Application to the absolute configuration determination of naturally occurring polyyne alcohols

   J. Chromatogr. A, 1136, 82-88 (2006)

C. Seger, K. Eberhart, S. Sturm, H. Strasser, H. Stuppner

Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography, High yield strategy for structurally closely related analytes - destruxin derivatives from Metarhizium anisopliae as a case study.

   J. Chromatogr. A, 1117, 67-73 (2006)

S. Schwaiger, C. Seger, B. Wiesbauer, P. Schneider, E. Ellmerer, S. Sturm, H. Stuppner

Development of a HPLC-DAD/MS assay for the Identification and Quantification of major phenolic Edelweiss (Leontopodium alpium Cass.) constituents.

   Phytochem. Anal., 17, 291-298 (2006)

J. Rollinger, J. Ewelt, C. Seger, S. Sturm, E. Ellmerer, H. Stuppner

New insights into the acetylcholinesterase inhibitory activity of Lycopodium clavatum L..

   Planta Med., 71, 1040-1043 (2005)

C. Seger, T. Längle, B. Pernfuss, H. Stuppner, H. Strasser

High-performance liquid chromatography-diode array detection assay for the detection and quantification of the Beauveria metabolite oosporein from potato tubers.

   J. Chromatogr. A 1092, 254-257 (2005)

T. Längle, B. Pernfuss, C. Seger, H. Strasser

Field efficacy evaluation of Beauveria brongniartii against Melolontha melolontha in potato cultures.

   Sydowia, 57, 54-97 (2005)

C. Seger, S. Sturm, E. Haslinger, H. Stuppner

NMR assignment of 22-deoxocucurbitacin D and cucurbitacin D from Ecballium elaterium L. (Cucurbitaceae).

   Monatsh. Chem.,136, 1645-1649 (2005)

J. Rollinger, A. Bodensieck, C. Seger, E. Ellmerer, R. Bauer, T. Langer, H. Stuppner

Discovering COX-inhibiting constituents of Morus root bark: Activity-guided versus computer-aided methods.

   Planta Med., 71, 399-405 (2005)

C. Seger, S. Sturm, M.E. Mair, E. Ellmerer, H. Stuppner

1H and 13C NMR signal assignment of cucurbitacine derivatives from Citrullus colocynthis (L.) Schrader and Ecballium elaterium L. (Cucurbitaceae).

   Magn. Reson. Chem., 43, 489-491 (2005)

C. Seger, D. Erlebach, H. Stuppner, U.J. Griesser, H. Strasser

Physico-chemical characterization of oosporein, a major metabolite of the entomopathogenic fungus Beauveria brongniartii.

   Helv. Chim. Acta, 88, 802-810 (2005)

S. Schwaiger, R. Cervellati, C. Seger, E. Ellmerer, N. About, I. Renimel, C.Godenir, P. André, H. Stuppner

Leontopodic acid – a novel highly substituted glucaric acid derivative from Edelweiss (Leontopodium alpinum Cass.) and its antioxidative and DNA protecting properties.

   Tetrahedron, 61, 4621-4630 (2005)

C. Seger, S. Sturm, T. Längle, W. Wimmer, H. Stuppner, H. Strasser

Development of a sensitive HPLC-DAD assay for the detection and quantification of the fungal metabolite oosporein from fungal culture broth and biological pest control formulations.

   J. Agri. Food Chem., 53, 1364 - 1369 (2005)

C. Seger, M. Godejohann, L.H. Tseng, M. Spraul, A. Girtler, S. Sturm, H. Stuppner

The LC-DAD-MS/SPE-NMR hyphenation. A tool for the analysis of pharmaceutically used plant extracts: Identification of isobaric iridoid glycoside regioisomers from Harpagophytum procumbens.

   Anal. Chem., 77, 878-885 (2005)

 

Publications before 2005

C. Seger, S. Sturm, H. Stuppner, T.M. Butt, H. Strasser

Combination of a new sample preparation strategy and an accelerated high-performance liquid chromatography assay with photodiode array and mass spectrometric detection for the determination of destruxins from Metarhizium anisopliae culture broth.

   J. Chromatogr. A 1061, 35-43 (2004)

S. Schwaiger, M. Adams, C. Seger, E. Ellmerer, R. Bauer, H. Stuppner

Novel constituents of Leontopodium alpinum Cass. and their in-vitro 5-Lipoxygenase inhibitory activity.

   Planta Med. 70, 978-985 (2004)

B. Brem, C. Seger, T. Pacher, M. Hartl, F. Hadacek, O. Hofer, S. Vajrodaya, H. Greger

Antioxidant dehydrotocopherols as a new chemical character of Stemona species.

   Phytochem. 65, 2719-2729 (2004)

C. Seger, S. Sturm, E.M. Strasser, E. Ellmerer, H. Stuppner

1H and 13C NMR signal assignment of benzyl-isoquinoline alkaloids from Fumaria officinalis L. (Papaveraceae).

   Magn. Reson. Chem. 42, 882-886 (2004)

R. Strommer, C. Hödl, W.L.S. Strauss, R. Sailer, E. Haslinger, H.W. Schramm, C. Seger

Synthesis of 6-hydroxy derivatives of steroidal hormones by SeO2 mediated oxidation.

   Monatsh. Chem. 135, 1137-1141 (2004)

C. Hödl, W.L.S. Strauss, R. Sailer,  C. Seger, R. Steiner, E. Haslinger, H.W. Schramm

A novel, highly affine, and fluorescent progesterone receptor antagonist. Synthesis and in vitro studies.

   Bioconjugate Chemistry, 15, 359-365 (2004)

A. Michelitsch, U. Rückert, A. Rittmannsberger, C. Seger, H. Strasser, W. Likussar

Accurate determination of oosporein in fungal culture broth by differential pulse polarography.

   J. Agri. Food Chem., 52, 1423-1426 (2004)

C. Seger, K. Mereiter, E. Kaltenegger, T. Pacher, H. Greger, O. Hofer

Two Pyrrolo[1,2-a]azepine type alkaloids from Stemona collinsae CRAIB: Structure elucidations, relationship to Asparagamine A, and a new biogenetic concept of their formation.

   Chemistry & Biodiversity, 1, 265-279 (2004)

C. Seger, H. Römpp, S. Sturm,  E. Haslinger, P.C. Schmidt, F. Hadacek

Characterization of supercritical fluid extracts of St. John's Wort (Hypericum perforatum L.) by HPLC-MS and GC-MS.

   Eur. J. Pharm. Sci., 21, 453-463 (2004)

H. Römpp,  C. Seger, C.S. Kaiser, E. Haslinger, P.C. Schmidt

Enrichment of hyperforin from St John's Wort (Hypericum perforatum) by pilot-scale supercritical carbon dioxide extraction.

   Eur. J. Pharm. Sci., 21, 443-451 (2004) 

C. Seger, S. Sturm, E. Haslinger, H. Stuppner

A new cucurbitacin D related 16,23-epoxy derivative and its isomerization products.

   Org. Lett., 6, 633-636 (2004)

C. Buchta, C. Bittner, P. Höcker, M. Macher, R. Schmid, C. Seger, M. Dettke

Donor exposure to the plasticizer di(2-ethylhexyl)phthalate during plateletpheresis.

   Transfusion, 43, 1115-1120 (2003)

B. Brem,  C. Seger, T. Pacher, O. Hofer, S. Vajrodaya, H. Greger

Stemona alkaloids – a source of potent natural insecticides.

   J. Agri. Food Chem., 50, 6383-6388 (2002)

H. A. Ridda Farid, O. Kunert, E. Haslinger, C. Seger

Isolation and structure elucidation of iridoide and coumarin derivatives from Xeromphis nilotica (Rubiaceae).

   Monatsh. Chem., 133, 1453-1458 (2002)

T. Pacher, C. Seger, S. Vajrodaya, O. Hofer, H. Greger

Antifungal stilbenoids from  Stemona collinsae.

   J. Nat. Prod., 65, 820-827 (2002)

A. Hiermann, S. Kedwani, H. W. Schramm, C. Seger

A new pyrrole alkaloid from seeds of Castanea sativa.

   Fitotherapia, 73, 22-27 (2002)

A. Hiermann, S. Kedwani, H. W. Schramm, C. Seger

Aglairubin: Structure revision of a chemotaxonomically interesting bisamide in Aglaia (Meliaceae).

   Monatsh. Chem., 133, 97-101 (2002)

B. Goger, O. Kunert, C. Seger, R. Rinelli, R. Wintersteiger

Quantification of phenyl urea pesticides by HPLC/ECD and photolysis.

   Electroanalysis, 13, 1335-1341 (2001)

A. Schwartz, C. Seger

Samarium(II) iodide promoted tandem reductive fragmentation and aldol reaction.

   Monatsh. Chem., 132, 855-858 (2001)

C. Seger, O. Hofer, H. Greger

Corrected structure of aglalactone isolated from Aglaia elaeagnoidea (Melicaceae).

   Monatsh. Chem., 131, 1161-1165 (2000)

R. Pfänder, L. Neumann, M. Zweckstetter, C. Seger, T. A. Holak, R. Tampe

Conformation of the active domain of the herpes simplex-virus Protein ICP47 in Water/SDS solution determined by NMR spectroscopy.

   Biochemistry, 38, 13692-13698 (1999)

C. Seger, S. Vajrodaya, H. Greger, O. Hofer

Structure elucidation and synthesis of a new bioactive quinazolone  derivative obtained from Glycosmis cf. chlorosperma.

   Chem. Pharm. Bull., 46, 1926-1928 (1998)

C. Seger, S. Vajrodaya, O. Hofer, H. Greger

Two new nor-diterpenes from Glycosmis cf. cyanocarpa.

   Nat. Prod. Lett., 12, 117-124 (1998)

C. Seger, B. Jandl, G. Brader, W. Robien, O. Hofer, H. Greger

Case studies of CSEARCH supported structure elucidation strategies: lupeol and a new germacrane derivative.

   Fresenius J. Anal. Chem., 359, 42-45 (1997)

 

Selected Conference Contributions

 

S. Sturm, C. Seger, M. Godejohann, M. Spraul, H. Stuppner

Biomarker identification from single plant tubers of corydalis solida by LC-SPE-NMR.

   Hyphenation 2006, 27-29 March 2006, Tübingen, Germany

C. Seger, M. Godejohann, M. Spraul, H. Stuppner, F. Hadacek

Reaction product analysis by HPLC-SPE-NMR: Application to the absolute configuration determination of naturally occurring polyyne alcohols.

   12th International Symposium on Separation Sciences, 27-29 September 2006, Lipica, Sloveniaustria

C. Seger, H. Stuppner

NMR based metabonomics and LC-SPE-NMR as novel analytical tools to unravel biological complexity.

   2nd Annual CMBI meeting, 31 September - 1 October 2005, Vill, Austria

K. Eberhart, C. Seger, S. Sturm, H. Strasser, H. Stuppner

High yield isolation of destruxins A, B, D, E, and E-diol, the major depsipeptide derivatives of Metarhizium anisopliae by combining lipophilic Sephadex gel chromatography and HSCCC.

   53rd GA Congress 21-25 August 2005, Florence, Italy

 M. Godejohann, C. Seger, L.H. Tseng, M. Spraul, A. Girtler, S. Sturm, H. Stuppner

Identification of iridoid glycoside regioisomers from Harpagophytum procumbens using a LC-DAD-MS/SPE-NMR hyphenation as dereplication tool.

   Joint Meeting DPhG, ÖPhG and CPS, 6-9 October 2004, Regensburg, Germany

 C. Seger, S. Sturm, H. Stupner, H. Strasser

A rapid method for the isolation and analysis of destruxins from Metarhizium anisopliae culture broth.

   15th Symposium on Pharmaceutical and Biomedical Analysis (PBA 2004) 2-6 May 2004, Florence, Italy

 

 

 

Update 2012-09-06 CS

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